Hotspot

rpoB-rps20

Genome · 41472-42493 · 1022 bp

Back to Porosira

Pi 0.261

MarkerSeek score 73.50

Primer available yes

Label rpoB-rps20

Feature Metadata

label_name: rpoB-rps20
feature_type: igs
region: Genome
length_bp: 1022
variable_sites: 147
pi: 0.261
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.55
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-rps20_p1	TCACTGGGAATTACGCGTTT	CTGCTCAACTAAAAGCTGCG	45.0	50.0	57.8	57.8	1491 bp	0.5	56.7
rpoB-rps20_p2	TTCACTGGGAATTACGCGTT	CTGCTCAACTAAAAGCTGCG	45.0	50.0	57.8	57.8	1492 bp	0.5	56.7
rpoB-rps20_p3	TTTCACTGGGAATTACGCGT	CTGCTCAACTAAAAGCTGCG	45.0	50.0	57.8	57.8	1493 bp	0.5	56.7
rpoB-rps20_p4	GGTTGTCCATCCCAATTCCA	CTGCTCAACTAAAAGCTGCG	50.0	50.0	57.8	57.8	1653 bp	0.5	56.3
rpoB-rps20_p5	TGGTTGTCCATCCCAATTCC	CTGCTCAACTAAAAGCTGCG	50.0	50.0	57.8	57.8	1654 bp	0.5	56.3