Hotspot

ycf1

Genome · 106836-110033 · 3198 bp

Back to Porella

Pi 0.025

MarkerSeek score 56.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 3198
variable_sites: 117
pi: 0.025
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCTTGCCAATACGGGACTTG	TACGGATTCCTCACCACACT	50.0	50.0	57.9	58.1	363 bp	1.0	83.2
ycf1_p2	CGAAAACTGCTACACTGGGT	TACGGATTCCTCACCACACT	50.0	50.0	58.1	58.1	856 bp	1.0	81.8
ycf1_p3	TCTTGCCAATACGGGACTTG	CTCACCACACTGCCCATTAG	50.0	55.0	57.9	58.3	354 bp	1.0	81.8
ycf1_p4	TCGAAAACTGCTACACTGGG	TACGGATTCCTCACCACACT	50.0	50.0	57.8	58.1	857 bp	1.0	81.4
ycf1_p5	CCGCAAAACCCACCTAGAAT	TACGGATTCCTCACCACACT	50.0	50.0	58.2	58.1	440 bp	0.333333	51.5