Hotspot

rps7

Genome · 1029-1496 · 468 bp

Back to Porella

Pi 0.0285

MarkerSeek score 59.20

Primer available yes

Label rps7

Feature Metadata

label_name: rps7
feature_type: gene
region: Genome
length_bp: 468
variable_sites: 20
pi: 0.0285
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps7_p1	AATCGGATGTACGGTTTGGG	TGGAAGACCCCAGTAAGGTT	50.0	50.0	58.0	57.9	967-1023 bp	0.666667	69.0
rps7_p2	AATCGGATGTACGGTTTGGG	TTGGAAGACCCCAGTAAGGT	50.0	50.0	58.0	57.9	968-1024 bp	0.666667	69.0
rps7_p3	AGGGGGACATTAGATGCTGT	TGGAAGACCCCAGTAAGGTT	50.0	50.0	58.1	57.9	1461-1520 bp	0.666667	67.8
rps7_p4	AGGGGGACATTAGATGCTGT	TTGGAAGACCCCAGTAAGGT	50.0	50.0	58.1	57.9	1462-1521 bp	0.666667	67.8
rps7_p5	CAAGGGGACTAAAGCATGCT	TGGAAGACCCCAGTAAGGTT	50.0	50.0	57.9	57.9	1309-1365 bp	0.666667	67.7