Hotspot

trnL-UAA-trnF-GAA

Genome · 52323-52419 · 97 bp

Back to Porella

Pi 0.055
MarkerSeek score 76.20
Primer available yes
Label trnL-UAA-trnF-GAA

Feature Metadata

label_name
trnL-UAA-trnF-GAA
feature_type
igs
region
Genome
length_bp
97
variable_sites
8
pi
0.055
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAA-trnF-GAA_p1 GTTCAAGTCCCTCTACCCCC AATCCCCTGCTCTACCAACT 60.0 50.0 59.1 58.0 151-154 bp 1.0 75.3
trnL-UAA-trnF-GAA_p2 GGGTTCAAGTCCCTCTACCC AATCCCCTGCTCTACCAACT 60.0 50.0 59.1 58.0 153-156 bp 1.0 75.2
trnL-UAA-trnF-GAA_p3 GGTTCAAGTCCCTCTACCCC AATCCCCTGCTCTACCAACT 60.0 50.0 59.1 58.0 152-155 bp 1.0 75.2
trnL-UAA-trnF-GAA_p4 GTTCAAGTCCCTCTACCCCC TCAATCCCCTGCTCTACCAA 60.0 50.0 59.1 58.0 153-156 bp 1.0 75.2
trnL-UAA-trnF-GAA_p5 GTTCAAGTCCCTCTACCCCC TTCAATCCCCTGCTCTACCA 60.0 50.0 59.1 58.0 154-157 bp 1.0 75.1