Hotspot

psbI-psbK

Genome · 23901-24232 · 332 bp

Back to Porella

Pi 0.0713

MarkerSeek score 80.80

Primer available yes

Label psbI-psbK

Feature Metadata

label_name: psbI-psbK
feature_type: igs
region: Genome
length_bp: 332
variable_sites: 34
pi: 0.0713
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-psbK_p1	GGATTACGTCCCGGATCATT	GCTTTTGTTTGGCAAGCTTC	50.0	45.0	57.2	57.0	415-465 bp	1.0	75.2
psbI-psbK_p2	GGATTACGTCCCGGATCATT	TTTGTTTGGCAAGCTTCTGT	50.0	40.0	57.2	56.7	412-462 bp	1.0	72.7
psbI-psbK_p3	GGATTACGTCCCGGATCATT	GCTTTTGTTTGGCAAGCTTCT	50.0	42.9	57.2	58.4	415-465 bp	1.0	71.8
psbI-psbK_p4	GGATTACGTCCCGGATCATT	TTTTGTTTGGCAAGCTTCTGT	50.0	38.1	57.2	57.4	413-463 bp	1.0	70.6
psbI-psbK_p5	GGATTACGTCCCGGATCATT	TGTTTGGCAAGCTTCTGTAAG	50.0	42.9	57.2	57.0	410-460 bp	1.0	67.6