Hotspot

trnS-GCU-psbI

Genome · 23755-23789 · 35 bp

Back to Porella

Pi 0.0762

MarkerSeek score 79.10

Primer available yes

Label trnS-GCU-psbI

Feature Metadata

label_name: trnS-GCU-psbI
feature_type: igs
region: Genome
length_bp: 35
variable_sites: 4
pi: 0.0762
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbI_p1	GCTATTCGTACCGAGGGTTC	CGTAATCCAGGACGTAAAGAGT	55.0	45.5	57.9	57.6	96 bp	1.0	63.3
trnS-GCU-psbI_p2	TGCTAATCCGTTGTACGAGC	CGTAATCCAGGACGTAAAGAGT	50.0	45.5	58.1	57.6	114 bp	1.0	63.2
trnS-GCU-psbI_p3	GGGTTCGAATCCCTCTCTCT	CGTAATCCAGGACGTAAAGAGT	55.0	45.5	58.2	57.6	82 bp	1.0	63.2
trnS-GCU-psbI_p4	AGCTATTCGTACCGAGGGTT	CGTAATCCAGGACGTAAAGAGT	50.0	45.5	58.2	57.6	97 bp	1.0	62.5
trnS-GCU-psbI_p5	GCTAATCCGTTGTACGAGCT	CGTAATCCAGGACGTAAAGAGT	50.0	45.5	57.8	57.6	113 bp	1.0	62.3