Hotspot

trnN-GUU-trnR-ACG

SSC · 130466-131138 · 673 bp

Back to Populus

Pi 0.012

MarkerSeek score 64.60

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: SSC
length_bp: 673
variable_sites: 40
pi: 0.012
unique_haplotype_count: 33
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	639-881 bp	1.0	86.1
trnN-GUU-trnR-ACG_p2	CCGTTGTTCCTCAGTAGCTC	TAGGGTATTCCGGGGAACAG	55.0	55.0	58.0	58.2	690-932 bp	0.982456	85.4
trnN-GUU-trnR-ACG_p3	CCGTTGTTCCTCAGTAGCTC	AACAGATCCAGTGGAGACGA	55.0	50.0	58.0	58.1	675-917 bp	0.947368	85.0
trnN-GUU-trnR-ACG_p4	GCTGTTAACTGACTGGTCGT	AACAGATCCAGTGGAGACGA	50.0	50.0	57.8	58.1	639-881 bp	0.947368	84.0
trnN-GUU-trnR-ACG_p5	TGGTCGTAGGTTCGAATCCT	AACAGATCCAGTGGAGACGA	50.0	50.0	58.2	58.1	626-868 bp	0.947368	84.0