Hotspot

ndhH-ycf1

SSC · 123778-124588 · 811 bp

Back to Populus

Pi 0.00823

MarkerSeek score 57.30

Primer available yes

Label ndhH-ycf1

Feature Metadata

label_name: ndhH-ycf1
feature_type: igs
region: SSC
length_bp: 811
variable_sites: 81
pi: 0.00823
unique_haplotype_count: 39
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-ycf1_p1	AAGGATGAGTCGAAGAACGC	ATCGAATCAACCCGTCTGTC	50.0	50.0	58.0	57.7	1286-1434 bp	1.0	87.3
ndhH-ycf1_p2	GATGAGTCGAAGAACGCCAT	ATCGAATCAACCCGTCTGTC	50.0	50.0	58.1	57.7	1283-1431 bp	1.0	86.8
ndhH-ycf1_p3	GGTCTGCCCACCAATATCTG	ATCGAATCAACCCGTCTGTC	55.0	50.0	58.0	57.7	1583-1731 bp	1.0	86.4
ndhH-ycf1_p4	AGCCATGAGGTAGATCAGCT	ATCGAATCAACCCGTCTGTC	50.0	50.0	57.9	57.7	1707-1855 bp	1.0	85.5
ndhH-ycf1_p5	ATTTCCCAACTGTTCCGGTC	ATCGAATCAACCCGTCTGTC	50.0	50.0	58.1	57.7	1469-1617 bp	0.982456	85.3