Hotspot

ccsA-ndhD

SSC · 115968-116193 · 226 bp

Back to Populus

Pi 0.0201
MarkerSeek score 60.60
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
226
variable_sites
26
pi
0.0201
unique_haplotype_count
35
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TGGATATGCTATTTTGGGGT CAGTTGACAAGGTCGAAGCT 40.0 50.0 54.0 58.1 339-428 bp 1.0 57.6
ccsA-ndhD_p2 TGGATATGCTATTTTGGGGT TCAGTTGACAAGGTCGAAGC 40.0 50.0 54.0 58.1 340-429 bp 1.0 57.5
ccsA-ndhD_p3 TGGATATGCTATTTTGGGGTT CAGTTGACAAGGTCGAAGCT 38.1 50.0 54.9 58.1 339-428 bp 1.0 56.7
ccsA-ndhD_p4 TGGATATGCTATTTTGGGGTT TCAGTTGACAAGGTCGAAGC 38.1 50.0 54.9 58.1 340-429 bp 1.0 56.7
ccsA-ndhD_p5 TGGATATGCTATTTTGGGGT TCGATTGCTATCCTTCTGCC 40.0 50.0 54.0 57.5 403-492 bp 1.0 53.8