Hotspot

ndhF-trnL-UAG

SSC · 114233-114798 · 566 bp

Back to Populus

Pi 0.0133
MarkerSeek score 58.90
Primer available yes
Label ndhF-trnL-UAG

Feature Metadata

label_name
ndhF-trnL-UAG
feature_type
igs
region
SSC
length_bp
566
variable_sites
41
pi
0.0133
unique_haplotype_count
41
primer_available
yes
alignment_reliability
0.94
markerseek_score
58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-trnL-UAG_p1 CGGAAAAAGTAGCAGTCCCA AGCGTGTCTACCGATTTCAC 50.0 50.0 57.8 58.0 347-767 bp 0.982456 86.8
ndhF-trnL-UAG_p2 CGGAAAAAGTAGCAGTCCCA CAGCGTGTCTACCGATTTCA 50.0 50.0 57.8 58.0 348-768 bp 0.982456 86.8
ndhF-trnL-UAG_p3 ACCGGAAAAAGTAGCAGTCC AGCGTGTCTACCGATTTCAC 50.0 50.0 57.8 58.0 349-769 bp 0.982456 86.8
ndhF-trnL-UAG_p4 ACCGGAAAAAGTAGCAGTCC CAGCGTGTCTACCGATTTCA 50.0 50.0 57.8 58.0 350-770 bp 0.982456 86.7
ndhF-trnL-UAG_p5 CGGAAAAAGTAGCAGTCCCA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.8 57.9 357-777 bp 0.982456 85.8