Hotspot

trnR-ACG-trnN-GUU

SSC · 109055-109731 · 677 bp

Back to Populus

Pi 0.0128

MarkerSeek score 65.10

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: SSC
length_bp: 677
variable_sites: 45
pi: 0.0128
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	639-872 bp	1.0	86.6
trnR-ACG-trnN-GUU_p2	TAGGGTATTCCGGGGAACAG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	58.2	58.0	690-923 bp	0.982456	85.9
trnR-ACG-trnN-GUU_p3	AACAGATCCAGTGGAGACGA	CCGTTGTTCCTCAGTAGCTC	50.0	55.0	58.1	58.0	675-908 bp	0.947368	85.4
trnR-ACG-trnN-GUU_p4	AACAGATCCAGTGGAGACGA	GCTGTTAACTGACTGGTCGT	50.0	50.0	58.1	57.8	639-872 bp	0.947368	84.4
trnR-ACG-trnN-GUU_p5	AACAGATCCAGTGGAGACGA	TGGTCGTAGGTTCGAATCCT	50.0	50.0	58.1	58.2	626-859 bp	0.947368	84.4