Hotspot

psbH-petD

LSC · 74857-77423 · 2567 bp

Back to Populus

Pi 0.00263

MarkerSeek score 59.80

Primer available yes

Label psbH-petD

Feature Metadata

label_name: psbH-petD
feature_type: igs
region: LSC
length_bp: 2567
variable_sites: 71
pi: 0.00263
unique_haplotype_count: 41
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH-petD_p1	GTCGCAATGGCTCTATTTGC	TGACCCATTCCTTTAGCCAA	50.0	45.0	57.9	56.7	2688-2800 bp	1.0	75.2
psbH-petD_p2	CTTTGATGGGTGTCGCAATG	TGACCCATTCCTTTAGCCAA	50.0	45.0	57.7	56.7	2699-2811 bp	1.0	73.9
psbH-petD_p3	GGTGTCGCAATGGCTCTATT	TGACCCATTCCTTTAGCCAA	50.0	45.0	58.3	56.7	2691-2803 bp	1.0	73.4
psbH-petD_p4	GTCGCAATGGCTCTATTTGC	ATGACCCATTCCTTTAGCCA	50.0	45.0	57.9	56.5	2689-2801 bp	1.0	73.3
psbH-petD_p5	GGTGGGGAACTACTCCTTTG	TGACCCATTCCTTTAGCCAA	55.0	45.0	57.5	56.7	2714-2826 bp	1.0	72.1