Hotspot

rps12-clpP

LSC · 69796-69988 · 193 bp

Back to Populus

Pi 0.0162

MarkerSeek score 59.10

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 193
variable_sites: 14
pi: 0.0162
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	ACCCTAGTACATGTCCCTCG	CAGAAGCCCAAGTTCATGGA	55.0	50.0	57.9	57.8	333-378 bp	1.0	85.2
rps12-clpP_p2	ACCCTAGTACATGTCCCTCG	TTATGTCAGCAGCAGAAGCC	55.0	50.0	57.9	58.3	345-390 bp	1.0	84.7
rps12-clpP_p3	ACCCTAGTACATGTCCCTCG	CACAAAGAACAGGCAAACCC	55.0	50.0	57.9	57.8	402-447 bp	1.0	84.5
rps12-clpP_p4	ACCCTAGTACATGTCCCTCG	TGCACAAAGAACAGGCAAAC	55.0	45.0	57.9	57.7	404-449 bp	1.0	84.0
rps12-clpP_p5	ACCCTAGTACATGTCCCTCG	GCACAAAGAACAGGCAAACC	55.0	50.0	57.9	58.4	403-448 bp	1.0	82.9