Hotspot

petA-psbJ

LSC · 62659-63603 · 945 bp

Back to Populus

Pi 0.00738

MarkerSeek score 67.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 945
variable_sites: 61
pi: 0.00738
unique_haplotype_count: 40
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCCGTTATTTTGGCAC	TATGGCTCCTATTCCGGCTT	50.0	50.0	58.1	58.3	1051-1120 bp	1.0	85.8
petA-psbJ_p2	GGCATCCGTTATTTTGGCAC	TTATGGCTCCTATTCCGGCT	50.0	50.0	58.1	58.3	1052-1121 bp	1.0	85.8
petA-psbJ_p3	GGCATCCGTTATTTTGGCAC	TATTCCGGCTTGGGTTCATC	50.0	50.0	58.1	57.7	1042-1111 bp	1.0	85.3
petA-psbJ_p4	GGCCTTTTGTTCTTCTTGGC	TATGGCTCCTATTCCGGCTT	50.0	50.0	57.8	58.3	1068-1137 bp	1.0	85.1
petA-psbJ_p5	GGCCTTTTGTTCTTCTTGGC	TTATGGCTCCTATTCCGGCT	50.0	50.0	57.8	58.3	1069-1138 bp	1.0	85.1