Hotspot

ndhC-trnV-UAC

LSC · 49856-51187 · 1332 bp

Back to Populus

Pi 0.0205

MarkerSeek score 73.70

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1332
variable_sites: 133
pi: 0.0205
unique_haplotype_count: 47
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGATAAAAGGCCCGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1304-1556 bp	1.0	89.3
ndhC-trnV-UAC_p2	AATTGGGGATAAAAGGCCCG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.2	57.8	1308-1560 bp	1.0	88.3
ndhC-trnV-UAC_p3	GGGGATAAAAGGCCCGAAAT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	1303-1555 bp	1.0	86.6
ndhC-trnV-UAC_p4	AATTGGGGATAAAAGGCCCG	GAAGGTCTACGGTTCGAGTC	50.0	55.0	58.2	57.5	1307-1559 bp	1.0	85.5
ndhC-trnV-UAC_p5	GGCCCGAAATTAGAAATGCC	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.5	57.8	1294-1546 bp	0.964912	84.4