Hotspot

psbZ-trnG-UCC

LSC · 35567-36072 · 506 bp

Back to Populus

Pi 0.018

MarkerSeek score 69.50

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 506
variable_sites: 50
pi: 0.018
unique_haplotype_count: 36
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	CGGTTGGTCAAGCAACAAAA	AAATCGACTTGGGCGGATAG	45.0	50.0	57.7	57.8	676-887 bp	0.982456	84.7
psbZ-trnG-UCC_p2	CGGTTGGTCAAGCAACAAAA	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.7	57.8	641-852 bp	0.947368	83.8
psbZ-trnG-UCC_p3	TTTCTTCTCCTGACGGTTGG	AAATCGACTTGGGCGGATAG	50.0	50.0	57.5	57.8	689-900 bp	1.0	83.4
psbZ-trnG-UCC_p4	TTTCTTCTCCTGACGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.5	57.8	654-865 bp	0.964912	82.5
psbZ-trnG-UCC_p5	TTCTTCTCCTGACGGTTGGT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.6	57.8	653-864 bp	0.964912	82.2