Hotspot

rps15-ycf1

SSC · 129862-130407 · 546 bp

Back to Pontederia

Pi 0.0942

MarkerSeek score 74.90

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 546
variable_sites: 170
pi: 0.0942
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AACACCATGCATTGATGGGT	AAGAAAAAGCGCCAAACACC	45.0	45.0	58.1	58.1	1317-1464 bp	0.769231	81.8
rps15-ycf1_p2	ACCATGCATTGATGGGTGTT	AAGAAAAAGCGCCAAACACC	45.0	45.0	58.1	58.1	1314-1461 bp	0.769231	81.8
rps15-ycf1_p3	CACCGTCGAGAGTAACGATC	AAGAAAAAGCGCCAAACACC	55.0	45.0	57.9	58.1	1345-1492 bp	0.769231	81.5
rps15-ycf1_p4	GGCTCAGCTCCAGCATAATT	AGGGGAGAAATGCGTCTAGA	50.0	50.0	58.0	57.8	1729-1882 bp	0.653846	76.7
rps15-ycf1_p5	GGCTCAGCTCCAGCATAATT	AAGAAAAAGCGCCAAACACC	50.0	45.0	58.0	58.1	1570-1717 bp	0.615385	75.9