Hotspot

rpl32-trnL-UAG

SSC · 119374-119965 · 592 bp

Back to Pontederia

Pi 0.0621

MarkerSeek score 83.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 592
variable_sites: 165
pi: 0.0621
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TTTTTGTGCGACAAACAAAT	TCCTAAGAGCAGCGTGTCTA	30.0	50.0	53.4	57.9	533-719 bp	0.961538	52.5
rpl32-trnL-UAG_p2	TTTTTGTGCGACAAACAAAT	AGCAGCGTGTCTACCAATTT	30.0	45.0	53.4	57.8	526-712 bp	0.961538	52.0
rpl32-trnL-UAG_p3	TTTTTGTGCGACAAACAAAT	CCTAAGAGCAGCGTGTCTAC	30.0	55.0	53.4	57.8	532-718 bp	0.961538	51.9
rpl32-trnL-UAG_p4	TTTTTGTGCGACAAACAAAT	CTAAGAGCAGCGTGTCTACC	30.0	55.0	53.4	57.8	531-717 bp	0.961538	51.9
rpl32-trnL-UAG_p5	TTTTTGTGCGACAAACAAAT	GAGCAGCGTGTCTACCAATT	30.0	50.0	53.4	58.3	527-713 bp	0.961538	51.3