Hotspot

petA-psbJ

LSC · 67843-69037 · 1195 bp

Back to Pontederia

Pi 0.0445

MarkerSeek score 90.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1195
variable_sites: 239
pi: 0.0445
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 90.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TTTGGCGCAAGTCTTTTTGG	CATATTCCGGCTTGGGTTCA	45.0	50.0	58.1	57.9	1209-1451 bp	0.846154	84.9
petA-psbJ_p2	TTTGGCGCAAGTCTTTTTGG	GTTCATATTCCGGCTTGGGT	45.0	50.0	58.1	57.9	1212-1454 bp	0.846154	84.9
petA-psbJ_p3	CCCATTACGTGTCCAAGGTC	CATATTCCGGCTTGGGTTCA	55.0	50.0	58.3	57.9	1195-1496 bp	0.884615	84.8
petA-psbJ_p4	GACCCATTACGTGTCCAAGG	CATATTCCGGCTTGGGTTCA	55.0	50.0	58.3	57.9	1197-1498 bp	0.884615	84.8
petA-psbJ_p5	CCCATTACGTGTCCAAGGTC	GTTCATATTCCGGCTTGGGT	55.0	50.0	58.3	57.9	1198-1499 bp	0.884615	84.8