Hotspot

psaI-ycf4

LSC · 65008-65399 · 392 bp

Back to Pontederia

Pi 0.0483

MarkerSeek score 88.40

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 392
variable_sites: 95
pi: 0.0483
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 88.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	ATTTCCGGCAATTGCAATGG	AAAAAGGATACAGGCCCAGC	45.0	50.0	58.0	58.1	535-546 bp	0.961538	87.4
psaI-ycf4_p2	GGCAATTGCAATGGCTTCTT	AAAAAGGATACAGGCCCAGC	45.0	50.0	57.9	58.1	529-540 bp	0.961538	86.8
psaI-ycf4_p3	ATTTCCGGCAATTGCAATGG	TTGTTTTTCGAGACCCCGTT	45.0	45.0	58.0	58.0	506-517 bp	0.923077	86.2
psaI-ycf4_p4	GGCAATTGCAATGGCTTCTT	TTGTTTTTCGAGACCCCGTT	45.0	45.0	57.9	58.0	500-511 bp	0.923077	85.7
psaI-ycf4_p5	GGCCTAGTATTTCCGGCAAT	TTGTTTTTCGAGACCCCGTT	50.0	45.0	57.7	58.0	514-525 bp	0.923077	84.3