Hotspot

accD-psaI

LSC · 63453-64896 · 1444 bp

Back to Pontederia

Pi 0.0498

MarkerSeek score 80.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 1444
variable_sites: 321
pi: 0.0498
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GTATGTGCTTCTGGAGGAGC	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.1	58.0	1726-2178 bp	0.961538	89.8
accD-psaI_p2	GTATGTGCTTCTGGAGGAGC	CACACGGAGCCTAGACAATC	55.0	55.0	58.1	58.1	1777-2229 bp	0.961538	89.5
accD-psaI_p3	GTATGTGCTTCTGGAGGAGC	AGACCTGCGATCCCATAGAA	55.0	50.0	58.1	57.9	2380-2838 bp	0.961538	89.3
accD-psaI_p4	TCAGCTCCACGGTTTCTTTC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	1376-1828 bp	0.961538	89.3
accD-psaI_p5	GTATGTGCTTCTGGAGGAGC	TTGTTTTTCGAGACCCCGTT	55.0	45.0	58.1	58.0	2241-2675 bp	0.923077	88.2