Hotspot

atpH-atpI

LSC · 15140-16903 · 1764 bp

Back to Pontederia

Pi 0.0686

MarkerSeek score 76.90

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1764
variable_sites: 439
pi: 0.0686
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACGGAAGCAGCAGAAATCA	CCATGGAGGGTCATCATTGA	45.0	50.0	58.1	57.0	1150-2484 bp	0.923077	79.8
atpH-atpI_p2	AACGGAAGCAGCAGAAATCA	TCCATGGAGGGTCATCATTG	45.0	50.0	58.1	57.0	1151-2485 bp	0.923077	79.8
atpH-atpI_p3	GCCAATCCAGCAGCAATAAC	CCATGGAGGGTCATCATTGA	50.0	50.0	57.8	57.0	1167-2501 bp	0.923077	78.9
atpH-atpI_p4	GCCAATCCAGCAGCAATAAC	TCCATGGAGGGTCATCATTG	50.0	50.0	57.8	57.0	1168-2502 bp	0.923077	78.9
atpH-atpI_p5	TCCAGCAGCAATAACGGAAG	CCATGGAGGGTCATCATTGA	50.0	50.0	58.3	57.0	1162-2496 bp	0.923077	78.5