Hotspot

rps15

SSC · 116585-116947 · 363 bp

Back to Pomatocalpa

Pi 0.482

MarkerSeek score 75.40

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 363
variable_sites: 146
pi: 0.482
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	CCCCAGACGTCGTAGAGAAT	AAATAAGGAACTGCGTGGCA	55.0	45.0	58.6	58.1	1401 bp	0.5	54.4
rps15_p2	CCCCAGACGTCGTAGAGAAT	TGTCCAATGTACCTTGTGGC	55.0	50.0	58.6	58.1	1167 bp	0.5	54.4
rps15_p3	ATTCTATCCCCCAGACGTCG	AAATAAGGAACTGCGTGGCA	55.0	45.0	58.7	58.1	1409 bp	0.5	53.9
rps15_p4	ATTCTATCCCCCAGACGTCG	TGTCCAATGTACCTTGTGGC	55.0	50.0	58.7	58.1	1175 bp	0.5	53.9
rps15_p5	CCCCAGACGTCGTAGAGAAT	CCAATGTACCTTGTGGCTCA	55.0	50.0	58.6	57.8	1164 bp	0.5	53.5