Hotspot

ndhD

SSC · 119268-119498 · 231 bp

Back to Pomatocalpa

Pi 0.433

MarkerSeek score 77.10

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 231
variable_sites: 94
pi: 0.433
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AGACTCCGAAAACCTCTGGA	AGGCATTCTTTCTGGAGCAG	50.0	50.0	58.0	57.9	1270 bp	0.5	58.9
ndhD_p2	TGGAGACTCCGAAAACCTCT	AGGCATTCTTTCTGGAGCAG	50.0	50.0	58.0	57.9	1273 bp	0.5	58.9
ndhD_p3	AGACTCCGAAAACCTCTGGA	GGCATTCTTTCTGGAGCAGT	50.0	50.0	58.0	58.2	1269 bp	0.5	58.6
ndhD_p4	TGGAGACTCCGAAAACCTCT	GGCATTCTTTCTGGAGCAGT	50.0	50.0	58.0	58.2	1272 bp	0.5	58.6
ndhD_p5	AGACTCCGAAAACCTCTGGA	TCTGGAGCAGTATGGGCTAA	50.0	50.0	58.0	57.8	1260 bp	0.5	58.5