Hotspot

ccsA

SSC · 120644-120919 · 276 bp

Back to Pomatocalpa

Pi 0.434

MarkerSeek score 75.40

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 276
variable_sites: 105
pi: 0.434
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TGGCGAAACCTTGGGTAAAA	CACGCTGCTCTTAGGAACTT	45.0	50.0	57.9	57.9	580 bp	0.5	58.8
ccsA_p2	GCGGGTTGATTTGCATTTCA	CACGCTGCTCTTAGGAACTT	45.0	50.0	57.9	57.9	616 bp	0.5	58.7
ccsA_p3	TGCGGGTTGATTTGCATTTC	CACGCTGCTCTTAGGAACTT	45.0	50.0	57.9	57.9	617 bp	0.5	58.7
ccsA_p4	TGGCGAAACCTTGGGTAAAA	AGACTCCGACTGAGAACGAA	45.0	50.0	57.9	58.1	881 bp	0.5	58.6
ccsA_p5	GCGGGTTGATTTGCATTTCA	AGACTCCGACTGAGAACGAA	45.0	50.0	57.9	58.1	917 bp	0.5	58.5