Hotspot

ndhD-ccsA

SSC · 119499-120643 · 1145 bp

Back to Pomatocalpa

Pi 0.46

MarkerSeek score 80.70

Primer available yes

Label ndhD-ccsA

Feature Metadata

label_name: ndhD-ccsA
feature_type: igs
region: SSC
length_bp: 1145
variable_sites: 446
pi: 0.46
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-ccsA_p1	AGACTCCGAAAACCTCTGGA	CACGCTGCTCTTAGGAACTT	50.0	50.0	58.0	57.9	2231 bp	0.5	59.2
ndhD-ccsA_p2	TGGAGACTCCGAAAACCTCT	CACGCTGCTCTTAGGAACTT	50.0	50.0	58.0	57.9	2234 bp	0.5	59.2
ndhD-ccsA_p3	AGACTCCGAAAACCTCTGGA	TAGACACGCTGCTCTTAGGA	50.0	50.0	58.0	57.9	2235 bp	0.5	58.9
ndhD-ccsA_p4	TGGAGACTCCGAAAACCTCT	TAGACACGCTGCTCTTAGGA	50.0	50.0	58.0	57.9	2238 bp	0.5	58.9
ndhD-ccsA_p5	TTGCATTGCCAGGAATGAGT	CACGCTGCTCTTAGGAACTT	45.0	50.0	58.1	57.9	1834 bp	0.5	58.7