Hotspot

psaC-ndhD

SSC · 118372-119267 · 896 bp

Back to Pomatocalpa

Pi 0.456

MarkerSeek score 83.20

Primer available yes

Label psaC-ndhD

Feature Metadata

label_name: psaC-ndhD
feature_type: igs
region: SSC
length_bp: 896
variable_sites: 383
pi: 0.456
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhD_p1	GTACCCAATGTGTTCGAGCT	ACTCATTCCTGGCAATGCAA	50.0	45.0	57.9	58.1	1024 bp	0.5	58.7
psaC-ndhD_p2	GTACCCAATGTGTTCGAGCT	ACCACTCATTCCTGGCAATG	50.0	50.0	57.9	58.2	1027 bp	0.5	58.0
psaC-ndhD_p3	TGTACCCAATGTGTTCGAGC	ACTCATTCCTGGCAATGCAA	50.0	45.0	58.2	58.1	1025 bp	0.5	57.8
psaC-ndhD_p4	GTACCCAATGTGTTCGAGCT	TCATTCCTGGCAATGCAAGA	50.0	45.0	57.9	57.8	1022 bp	0.5	57.5
psaC-ndhD_p5	TGTACCCAATGTGTTCGAGC	ACCACTCATTCCTGGCAATG	50.0	50.0	58.2	58.2	1028 bp	0.5	57.2