Hotspot

rps15-ndhG

SSC · 116948-117053 · 106 bp

Back to Pomatocalpa

Pi 0.424

MarkerSeek score 75.00

Primer available yes

Label rps15-ndhG

Feature Metadata

label_name: rps15-ndhG
feature_type: igs
region: SSC
length_bp: 106
variable_sites: 39
pi: 0.424
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ndhG_p1	ATCACAAAGGGGTCTACGAA	AAATAAGGAACTGCGTGGCA	45.0	45.0	56.2	58.1	519 bp	0.5	44.6
rps15-ndhG_p2	TCACAAAGGGGTCTACGAAT	AAATAAGGAACTGCGTGGCA	45.0	45.0	56.2	58.1	518 bp	0.5	44.6
rps15-ndhG_p3	TGTGCATTAGGAAGAGTGGA	AAATAAGGAACTGCGTGGCA	45.0	45.0	56.2	58.1	962 bp	0.5	44.5
rps15-ndhG_p4	ATCACAAAGGGGTCTACGAA	CCGTTCATAAACAGCACAGC	45.0	50.0	56.2	57.8	569 bp	0.5	43.4
rps15-ndhG_p5	TCACAAAGGGGTCTACGAAT	CCGTTCATAAACAGCACAGC	45.0	50.0	56.2	57.8	568 bp	0.5	43.4