Hotspot

ycf1-rps15

SSC · 116135-116584 · 450 bp

Back to Pomatocalpa

Pi 0.481

MarkerSeek score 76.30

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: SSC
length_bp: 450
variable_sites: 215
pi: 0.481
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	TTGCACGACACTAGCAACAT	AAATAAGGAACTGCGTGGCA	45.0	45.0	58.1	58.1	1484 bp	0.5	58.3
ycf1-rps15_p2	TTGCACGACACTAGCAACAT	TGTCCAATGTACCTTGTGGC	45.0	50.0	58.1	58.1	1250 bp	0.5	58.3
ycf1-rps15_p3	TTGCACGACACTAGCAACAT	CCAATGTACCTTGTGGCTCA	45.0	50.0	58.1	57.8	1247 bp	0.5	57.4
ycf1-rps15_p4	TTGCACGACACTAGCAACAT	GTCCAATGTACCTTGTGGCT	45.0	50.0	58.1	57.8	1249 bp	0.5	57.4
ycf1-rps15_p5	TTGCACGACACTAGCAACAT	CCGTTCATAAACAGCACAGC	45.0	50.0	58.1	57.8	1534 bp	0.5	57.0