Hotspot

psbK-psbI

LSC · 7726-8210 · 485 bp

Back to Pomatocalpa

Pi 0.0252

MarkerSeek score 72.60

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 485
variable_sites: 12
pi: 0.0252
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	GCCCCGGATCGTTAGATAAG	50.0	55.0	57.9	57.6	594-603 bp	1.0	79.4
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TTCTTCACGCCCAGGATTAC	50.0	50.0	57.9	57.6	614-623 bp	1.0	79.1
psbK-psbI_p3	TGGCAAGCTGCTGTAAGTTT	GCCCCGGATCGTTAGATAAG	45.0	55.0	58.3	57.6	591-600 bp	1.0	77.4
psbK-psbI_p4	TTGGCAAGCTGCTGTAAGTT	GCCCCGGATCGTTAGATAAG	45.0	55.0	58.3	57.6	592-601 bp	1.0	77.4
psbK-psbI_p5	TTTGGCAAGCTGCTGTAAGT	GCCCCGGATCGTTAGATAAG	45.0	55.0	58.3	57.6	593-602 bp	1.0	77.4