Hotspot

ndhI

SSC · 114102-114617 · 516 bp

Back to Polystichum

Pi 0.268

MarkerSeek score 82.90

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 516
variable_sites: 269
pi: 0.268
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	TTTTGCGCCCTCTCTTTTCT	TAGGTAGCAGGTTGGGTTCA	45.0	50.0	58.0	58.0	657-661 bp	0.5	64.0
ndhI_p2	CTCCTGAAGCGGTGAAGTAC	GGTGAGTCCTTTGACGTGTT	55.0	50.0	58.0	58.1	1323-1335 bp	0.5	63.8
ndhI_p3	CTCCTGAAGCGGTGAAGTAC	TAGGTAGCAGGTTGGGTTCA	55.0	50.0	58.0	58.0	1004-1008 bp	0.5	63.4
ndhI_p4	TGAAAACCAACCCCGAAGAG	TAGGTAGCAGGTTGGGTTCA	50.0	50.0	58.0	58.0	830-834 bp	0.5	63.4
ndhI_p5	CGCCCTCTCTTTTCTCTTCC	TAGGTAGCAGGTTGGGTTCA	55.0	50.0	58.0	58.0	656 bp	0.25	49.6