Hotspot

chlN

SSC · 124170-125555 · 1386 bp

Back to Polystichum

Pi 0.228

MarkerSeek score 83.90

Primer available yes

Label chlN

Feature Metadata

label_name: chlN
feature_type: gene
region: SSC
length_bp: 1386
variable_sites: 600
pi: 0.228
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlN_p1	CTATCTCTTCGCGCTTCTCC	GTGTGGCCCGAAGATGTAAT	55.0	50.0	58.0	58.0	2461-2469 bp	0.5	64.1
chlN_p2	CTATCTCTTCGCGCTTCTCC	ATCTCGAGGGTGAAGGGAAA	55.0	50.0	58.0	58.0	2040 bp	0.25	49.3
chlN_p3	CAGGTAGTGTCGCGAGAAAA	GTGTGGCCCGAAGATGTAAT	50.0	50.0	57.9	58.0	2613 bp	0.25	49.2
chlN_p4	CTATCTCTTCGCGCTTCTCC	CTAAGCCAGAGGGAGTCGTA	55.0	55.0	58.0	57.9	1937 bp	0.25	49.2
chlN_p5	AGGTAGTGTCGCGAGAAAAC	GTGTGGCCCGAAGATGTAAT	50.0	50.0	57.9	58.0	2612 bp	0.25	49.2