Hotspot

trnN-GUU-trnH-GUG

IRa · 127023-134118 · 7096 bp

Back to Polystichum

Pi 0.0277

MarkerSeek score 60.90

Primer available yes

Label trnN-GUU-trnH-GUG

Feature Metadata

label_name: trnN-GUU-trnH-GUG
feature_type: igs
region: IRa
length_bp: 7096
variable_sites: 334
pi: 0.0277
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnH-GUG_p1	TGGGTAGTTGTGTTTCCCAC	GTCACTGGATCAAACGACGA	50.0	50.0	57.6	57.9	262-556 bp	0.75	75.4
trnN-GUU-trnH-GUG_p2	TGGGTAGTTGTGTTTCCCAC	GAAACCGTTGCCCATTTCAG	50.0	50.0	57.6	57.9	353-647 bp	0.75	74.9
trnN-GUU-trnH-GUG_p3	TGGGTAGTTGTGTTTCCCAC	TTCCATCCGTTCTGGTTTGG	50.0	50.0	57.6	58.1	288-582 bp	0.75	74.9
trnN-GUU-trnH-GUG_p4	TGGGTAGTTGTGTTTCCCAC	AATCTCCCCTTGGGTGAAGA	50.0	50.0	57.6	57.9	540-834 bp	0.75	74.1
trnN-GUU-trnH-GUG_p5	TGGGTAGTTGTGTTTCCCAC	AGTCCCGCTCACTTTGATTC	50.0	50.0	57.6	57.9	450-744 bp	0.75	74.1