Hotspot

ndhE-ndhI

SSC · 113261-114101 · 841 bp

Back to Polystichum

Pi 0.267

MarkerSeek score 84.20

Primer available yes

Label ndhE-ndhI

Feature Metadata

label_name: ndhE-ndhI
feature_type: igs
region: SSC
length_bp: 841
variable_sites: 425
pi: 0.267
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhI_p1	CACTGCGTACAACCGATACA	CTTGCGAAGTATGTGTCCGA	50.0	50.0	58.0	58.0	1839-1844 bp	0.5	63.5
ndhE-ndhI_p2	CACTGCGTACAACCGATACA	TTGCGAAGTATGTGTCCGAG	50.0	50.0	58.0	58.0	1838-1843 bp	0.5	63.5
ndhE-ndhI_p3	CACTGCGTACAACCGATACA	TGTCCGAGTATGTCCGATCA	50.0	50.0	58.0	58.0	1826-1831 bp	0.5	63.2
ndhE-ndhI_p4	CACTGCGTACAACCGATACA	CGATCAATCTGCCGGTTGTA	50.0	50.0	58.0	58.1	1812-1817 bp	0.5	63.1
ndhE-ndhI_p5	CACTGCGTACAACCGATACA	TAGGTAGCAGGTTGGGTTCA	50.0	50.0	58.0	58.0	2063 bp	0.25	49.6