Hotspot

ccsA-ndhD

SSC · 110338-110735 · 398 bp

Back to Polystichum

Pi 0.265

MarkerSeek score 76.70

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 398
variable_sites: 175
pi: 0.265
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TCCACGCAATTAGTACCTGC	CAGGAATGAGCGGTTTCGTA	50.0	50.0	58.0	58.0	1303-1321 bp	0.5	66.3
ccsA-ndhD_p2	TCCACGCAATTAGTACCTGC	CTTCTTTCGATCGTGGCTGA	50.0	50.0	58.0	58.0	1665 bp	0.25	49.8
ccsA-ndhD_p3	TCCACGCAATTAGTACCTGC	TCTTCTTTCGATCGTGGCTG	50.0	50.0	58.0	58.0	1666 bp	0.25	49.8
ccsA-ndhD_p4	GGTCGGTGATGCATGTAACT	CTTCTTTCGATCGTGGCTGA	50.0	50.0	58.0	58.0	1631 bp	0.25	49.8
ccsA-ndhD_p5	GGTCGGTGATGCATGTAACT	TCTTCTTTCGATCGTGGCTG	50.0	50.0	58.0	58.0	1632 bp	0.25	49.8