Hotspot

trnP-GGG-trnL-UAG

SSC · 108735-109016 · 282 bp

Back to Polystichum

Pi 0.343
MarkerSeek score 75.90
Primer available yes
Label trnP-GGG-trnL-UAG

Feature Metadata

label_name
trnP-GGG-trnL-UAG
feature_type
igs
region
SSC
length_bp
282
variable_sites
168
pi
0.343
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.91
markerseek_score
75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-GGG-trnL-UAG_p1 GATTCGAACCTGTGACCTCC CTGGCACTGCTTCCTAAGAG 55.0 55.0 58.0 58.0 359-394 bp 0.75 80.8
trnP-GGG-trnL-UAG_p2 GATTCGAACCTGTGACCTCC CTCTGGCACTGCTTCCTAAG 55.0 55.0 58.0 58.0 361-396 bp 0.75 80.7
trnP-GGG-trnL-UAG_p3 GGATTCGAACCTGTGACCTC CTCTGGCACTGCTTCCTAAG 55.0 55.0 58.0 58.0 362-397 bp 0.75 80.7
trnP-GGG-trnL-UAG_p4 GGATTCGAACCTGTGACCTC CTGGCACTGCTTCCTAAGAG 55.0 55.0 58.0 58.0 360-395 bp 0.75 80.7
trnP-GGG-trnL-UAG_p5 GATTCGAACCTGTGACCTCC AGAAACATCGACGACTCGTG 55.0 50.0 58.0 58.0 916-947 bp 0.5 68.6