Hotspot

rpl32-trnP-GGG

SSC · 108015-108660 · 646 bp

Back to Polystichum

Pi 0.309

MarkerSeek score 84.40

Primer available yes

Label rpl32-trnP-GGG

Feature Metadata

label_name: rpl32-trnP-GGG
feature_type: igs
region: SSC
length_bp: 646
variable_sites: 362
pi: 0.309
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnP-GGG_p1	TAATCGCGCGTGGAAAACTA	CTCTGGCACTGCTTCCTAAG	45.0	55.0	58.0	58.0	1157-1189 bp	0.75	81.0
rpl32-trnP-GGG_p2	TAATCGCGCGTGGAAAACTA	CTGGCACTGCTTCCTAAGAG	45.0	55.0	58.0	58.0	1155-1187 bp	0.75	81.0
rpl32-trnP-GGG_p3	TAATCGCGCGTGGAAAACTA	GAGGTCACAGGTTCGAATCC	45.0	55.0	58.0	58.0	798-847 bp	0.75	78.8
rpl32-trnP-GGG_p4	TAATCGCGCGTGGAAAACTA	GGAGGTCACAGGTTCGAATC	45.0	55.0	58.0	58.0	799-848 bp	0.75	78.8
rpl32-trnP-GGG_p5	ACGGATCTGGCATCAACTTC	GAGGTCACAGGTTCGAATCC	50.0	55.0	58.0	58.0	1234-1283 bp	0.5	66.9