Hotspot

trnS-GCU-ycf12

LSC · 7615-8192 · 578 bp

Back to Polystichum

Pi 0.0715

MarkerSeek score 83.60

Primer available yes

Label trnS-GCU-ycf12

Feature Metadata

label_name: trnS-GCU-ycf12
feature_type: igs
region: LSC
length_bp: 578
variable_sites: 77
pi: 0.0715
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-ycf12_p1	TGCAGGCGGAGATATCAGTA	AATGGTCCCGATGCAACTAC	50.0	50.0	58.0	58.0	1268-1305 bp	1.0	90.0
trnS-GCU-ycf12_p2	TGCAGGCGGAGATATCAGTA	ACTAATGGTCCCGATGCAAC	50.0	50.0	58.0	58.0	1271-1308 bp	1.0	90.0
trnS-GCU-ycf12_p3	TGCAGGCGGAGATATCAGTA	TTCACGGTTATGCTGCCTAC	50.0	50.0	58.0	58.0	1348 bp	0.5	59.9
trnS-GCU-ycf12_p4	TGCAGGCGGAGATATCAGTA	CACGGTTATGCTGCCTACAT	50.0	50.0	58.0	58.0	1346 bp	0.5	59.7
trnS-GCU-ycf12_p5	GGGATTTGAACCCTCGGTAC	TTCACGGTTATGCTGCCTAC	55.0	50.0	58.0	58.0	779 bp	0.5	59.7