Hotspot

psbK-psbI

LSC · 6787-7342 · 556 bp

Back to Polystichum

Pi 0.065

MarkerSeek score 84.20

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 556
variable_sites: 64
pi: 0.065
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CGCAGTTAGCTTCCGGTAA	ACAAATAGTTTGAGGGTAAGCA	52.6	36.4	57.6	55.6	583-602 bp	1.0	50.2
psbK-psbI_p2	CGCAGTTAGCTTCCGGTAA	AGACAAATAGTTTGAGGGTAAGCA	52.6	37.5	57.6	58.1	585-604 bp	1.0	50.1
psbK-psbI_p3	CGCAGTTAGCTTCCGGTAA	TGCATAGACAAATAGTTTGAGGGT	52.6	37.5	57.6	58.2	590-609 bp	1.0	50.1
psbK-psbI_p4	CGCAGTTAGCTTCCGGTAA	GACAAATAGTTTGAGGGTAAGCA	52.6	39.1	57.6	56.8	584-603 bp	1.0	50.1
psbK-psbI_p5	CGCAGTTAGCTTCCGGTAA	CTGCATAGACAAATAGTTTGAGGG	52.6	41.7	57.6	57.6	591-610 bp	1.0	50.1