Hotspot

ndhI-rps15

SSC · 115504-116748 · 1245 bp

Back to Polystachya

Pi 0.0505

MarkerSeek score 75.10

Primer available yes

Label ndhI-rps15

Feature Metadata

label_name: ndhI-rps15
feature_type: igs
region: SSC
length_bp: 1245
variable_sites: 109
pi: 0.0505
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-rps15_p1	CCGTTCATAAACATCGCAGC	GGCAATTTTTGAATGAGGGCA	50.0	42.9	57.9	57.9	2233 bp	0.2	38.8
ndhI-rps15_p2	ACCCGTTCATAAACATCGCA	GGCAATTTTTGAATGAGGGCA	45.0	42.9	57.9	57.9	2235 bp	0.2	38.7
ndhI-rps15_p3	CTTGCGGCTCGTATTGTTTG	GGCAATTTTTGAATGAGGGCA	50.0	42.9	58.1	57.9	1940 bp	0.2	38.6
ndhI-rps15_p4	AAAATTCCGGACCATGACGT	GGCAATTTTTGAATGAGGGCA	45.0	42.9	57.8	57.9	2355 bp	0.2	38.0
ndhI-rps15_p5	TTCCGGACCATGACGTATCT	GGCAATTTTTGAATGAGGGCA	50.0	42.9	58.2	57.9	2351 bp	0.2	37.7