Hotspot

trnL-UAG-ccsA

SSC · 110787-110927 · 141 bp

Back to Polystachya

Pi 0.0726

MarkerSeek score 77.80

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 141
variable_sites: 18
pi: 0.0726
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	TAGACACGCTGCTCTTAGGA	GTCCCGAAGAAATCCAACGA	50.0	50.0	57.9	57.9	389-404 bp	1.0	86.4
trnL-UAG-ccsA_p2	TAAGCCGCCATGGTGAAATT	GTCCCGAAGAAATCCAACGA	45.0	50.0	58.2	57.9	411-426 bp	1.0	85.7
trnL-UAG-ccsA_p3	TAGACACGCTGCTCTTAGGA	CTGCTCCAGAAAGAATGCCT	50.0	50.0	57.9	57.9	920-935 bp	1.0	82.7
trnL-UAG-ccsA_p4	TAGACACGCTGCTCTTAGGA	TGGCGAAACCTTGGGTAAAA	50.0	45.0	57.9	57.9	536-546 bp	0.8	75.9
trnL-UAG-ccsA_p5	TAAGCCGCCATGGTGAAATT	TGGCGAAACCTTGGGTAAAA	45.0	45.0	58.2	57.9	558-568 bp	0.8	75.5