Hotspot

rpoA-rps11

LSC · 78254-78345 · 92 bp

Back to Polystachya

Pi 0.0522
MarkerSeek score 79.30
Primer available yes
Label rpoA-rps11

Feature Metadata

label_name
rpoA-rps11
feature_type
igs
region
LSC
length_bp
92
variable_sites
9
pi
0.0522
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoA-rps11_p1 AAGTGGGGACAGAACGAAAC GGAACACCTTATGCTGCTCA 50.0 50.0 58.0 57.9 415-424 bp 1.0 83.0
rpoA-rps11_p2 TCGCATTTCGAACAGGCATA GGAACACCTTATGCTGCTCA 45.0 50.0 58.0 57.9 851-860 bp 1.0 81.6
rpoA-rps11_p3 TCGCATTTCGAACAGGCATA AAGAGATGCGGCATTACGAG 45.0 50.0 58.0 58.1 747-756 bp 1.0 81.5
rpoA-rps11_p4 GTGTCCTCTTCCTCTGCATG GGAACACCTTATGCTGCTCA 55.0 50.0 58.0 57.9 1014-1023 bp 1.0 81.3
rpoA-rps11_p5 GTGTCCTCTTCCTCTGCATG AAGAGATGCGGCATTACGAG 55.0 50.0 58.0 58.1 910-919 bp 1.0 81.2