Hotspot

psbB-psbT

LSC · 72961-73367 · 407 bp

Back to Polystachya

Pi 0.0548

MarkerSeek score 82.10

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 407
variable_sites: 44
pi: 0.0548
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	GGAGATCCAACTACAAGGAGAC	GGAATGTATAAACCAATGCTTCCA	50.0	37.5	57.6	57.5	428-555 bp	1.0	51.4
psbB-psbT_p2	AGGAGATCCAACTACAAGGAGA	GGAATGTATAAACCAATGCTTCCA	45.5	37.5	57.7	57.5	429-556 bp	1.0	51.3
psbB-psbT_p3	AGGAGATCCAACTACAAGGAGA	AGGAATGTATAAACCAATGCTTCC	45.5	37.5	57.7	57.3	430-557 bp	1.0	51.3
psbB-psbT_p4	TGCTCAAGTAGAATTTGGAGCA	GGAATGTATAAACCAATGCTTCCA	40.9	37.5	58.0	57.5	462-589 bp	1.0	50.9
psbB-psbT_p5	TGCTCAAGTAGAATTTGGAGCA	AGGAATGTATAAACCAATGCTTCC	40.9	37.5	58.0	57.3	463-590 bp	1.0	50.9