Hotspot

trnM-CAU-atpE

LSC · 50306-50574 · 269 bp

Back to Polystachya

Pi 0.0698

MarkerSeek score 79.80

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 269
variable_sites: 32
pi: 0.0698
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	TATTGCTTTCATACGGCGGG	CTCTCAGACGAGCTAGGACA	50.0	55.0	58.4	58.0	304-466 bp	1.0	87.3
trnM-CAU-atpE_p2	TATTGCTTTCATACGGCGGG	CGAGCTAGGACACGAGTAGA	50.0	55.0	58.4	58.1	296-458 bp	1.0	87.2
trnM-CAU-atpE_p3	TATTGCTTTCATACGGCGGG	AGGACACGAGTAGAAGCTGT	50.0	50.0	58.4	58.1	290-452 bp	1.0	87.1
trnM-CAU-atpE_p4	TATTGCTTTCATACGGCGGG	GGACACGAGTAGAAGCTGTC	50.0	55.0	58.4	57.7	289-451 bp	1.0	85.7
trnM-CAU-atpE_p5	TATTGCTTTCATACGGCGGG	TCTCAGACGAGCTAGGACAC	50.0	55.0	58.4	58.3	303-465 bp	1.0	85.5