Pi
0.0494
MarkerSeek score
85.20
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 700-769 bp | 1.0 | 88.1 |
| trnT-UGU-trnL-UAA_p2 | GGAATCGAACCGATGACCAT | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 57.8 | 58.1 | 708-777 bp | 1.0 | 87.5 |
| trnT-UGU-trnL-UAA_p3 | CTAACCTCTGAGCTAAGCGG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.5 | 58.0 | 660-729 bp | 1.0 | 86.3 |
| trnT-UGU-trnL-UAA_p4 | GGAATCGAACCGATGACCAT | ATCCAATCAAGTCCGTAGCG | 50.0 | 50.0 | 57.8 | 57.8 | 723-792 bp | 1.0 | 86.1 |
| trnT-UGU-trnL-UAA_p5 | GGAATCGAACCGATGACCAT | AGTCCGTAGCGTCTACCAAT | 50.0 | 50.0 | 57.8 | 58.2 | 714-783 bp | 1.0 | 86.1 |