Hotspot

trnG-UCC-trnR-UCU

LSC · 10295-10450 · 156 bp

Back to Polystachya

Pi 0.0591

MarkerSeek score 82.90

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 156
variable_sites: 17
pi: 0.0591
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	210-243 bp	1.0	73.3
trnG-UCC-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.1	56.9	213-246 bp	1.0	73.0
trnG-UCC-trnR-UCU_p3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	216-249 bp	1.0	72.4
trnG-UCC-trnR-UCU_p4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	55.0	45.5	58.1	57.9	219-252 bp	1.0	72.1
trnG-UCC-trnR-UCU_p5	CCAACGTCGACTATAACCCC	AGAAGACCTCTGTCCTATCCA	55.0	47.6	57.8	56.9	231-264 bp	1.0	71.3