Hotspot

ycf1

SSC · 126134-131755 · 5622 bp

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Pi 0.0055
MarkerSeek score 42.80
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5622
variable_sites
99
pi
0.0055
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 ATGCTTGTGACAAGGAGGAT ACGCAGATATCCTTCTATCT 45.0 40.0 56.8 52.7 225-237 bp 1.0 46.7
ycf1_p2 TATGCTTGTGACAAGGAGGA ACGCAGATATCCTTCTATCT 45.0 40.0 56.2 52.7 226-238 bp 1.0 46.7
ycf1_p3 TGCTTGTGACAAGGAGGATA ACGCAGATATCCTTCTATCT 45.0 40.0 56.2 52.7 224-236 bp 1.0 46.7
ycf1_p4 GCTTGTGACAAGGAGGATAAGT ACGCAGATATCCTTCTATCT 45.5 40.0 58.1 52.7 223-235 bp 1.0 46.7
ycf1_p5 TGCTTGTGACAAGGAGGATAAG ACGCAGATATCCTTCTATCT 45.5 40.0 58.1 52.7 224-236 bp 1.0 46.7