Hotspot

rps16

LSC · 5058-6178 · 1121 bp

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Pi 0.00411

MarkerSeek score 38.00

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1121
variable_sites: 11
pi: 0.00411
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ACCAAACCTATGGACGGAGA	TCCATATCCATACCGGCTACA	50.0	47.6	58.1	57.8	1664-1667 bp	1.0	71.4
rps16_p2	ACTTGTGTTGGATTGGCACT	TCCATATCCATACCGGCTACA	45.0	47.6	57.9	57.8	1537-1540 bp	1.0	71.2
rps16_p3	GTTGGATTGGCACTCCATCT	TCCATATCCATACCGGCTACA	50.0	47.6	57.9	57.8	1531-1534 bp	1.0	70.7
rps16_p4	TGTTGGATTGGCACTCCATC	TCCATATCCATACCGGCTACA	50.0	47.6	58.2	57.8	1532-1535 bp	1.0	70.5
rps16_p5	TCACTCCCCCACCTTTTGTA	TCCATATCCATACCGGCTACA	50.0	47.6	58.2	57.8	1182-1186 bp	1.0	70.0