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rpl20-rps12

LSC · 71106-71885 · 780 bp

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Pi 0.00363

MarkerSeek score 50.70

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 780
variable_sites: 9
pi: 0.00363
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CTTGAATGAGCCCCTCGAAA	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	936-937 bp	1.0	75.5
rpl20-rps12_p2	TTGAATGAGCCCCTCGAAAG	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	935-936 bp	1.0	75.5
rpl20-rps12_p3	TCCCCGTCTAATTCTGGTCA	AAATGTCACGAAATCCCCCG	50.0	50.0	57.8	58.5	868-869 bp	1.0	74.6
rpl20-rps12_p4	CTTGAATGAGCCCCTCGAAA	CAGCGTCGAGGAACATGTAC	50.0	55.0	57.9	58.7	899-900 bp	1.0	74.0
rpl20-rps12_p5	TTGAATGAGCCCCTCGAAAG	CAGCGTCGAGGAACATGTAC	50.0	55.0	57.9	58.7	898-899 bp	1.0	74.0