Hotspot

petN-psbM

LSC · 29550-30632 · 1083 bp

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Pi 0.00441

MarkerSeek score 44.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1083
variable_sites: 14
pi: 0.00441
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1184-1190 bp	1.0	66.9
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTC	50.0	45.0	57.6	56.1	1184-1190 bp	1.0	62.7
petN-psbM_p3	TGGGGAAGAAGTGGACTCTA	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.0	57.6	1183-1189 bp	1.0	61.6
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	GCTACTGCACTGTTCATTCT	50.0	45.0	57.6	55.8	1183-1189 bp	1.0	60.3
petN-psbM_p5	TGGGGAAGAAGTGGACTCTAG	TGCTACTGCACTGTTCATTCT	52.4	42.9	58.2	57.6	1183-1189 bp	1.0	60.3